| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174173 |
| Chromosome: | chromosome 3 |
| Location: | 2532437 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160100 | (1 of 1) K06889 - uncharacterized protein (K06889) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTTTTTTGTTCCATAAAATACCCTGC |
| Internal bar code: | TGTGATCCCGAAGAGCGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGATCTGCTCCTTGG |
| Suggested primer 2: | TATGACGTGCCTAACCCCTC |