| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174178 |
| Chromosome: | chromosome 7 |
| Location: | 394215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314866 | EBM4 | (1 of 9) 3.2.1.78 - Mannan endo-1,4-beta-mannosidase / Endo-1,4-mannanase; Mannan endo-1%252C4-beta-mannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCCGTGGACTCCATTGCTACGTGTGTT |
| Internal bar code: | AGGTGCGTAAATCTATTTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 99.771 |
| LEAP-Seq n confirming: | 2178 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGTACACCACCCCCA |
| Suggested primer 2: | CCACAAGTTCTACGGCCATT |