Insertion junction: LMJ.RY0402.174196_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.174196
Chromosome:chromosome 4
Location:1709551
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g212600 (1 of 15) IPR029057 - Phosphoribosyltransferase-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGTCGGTTCGTAAGCTCGCTCCATACC
Internal bar code:AGGGCTCTGAACCGTAGAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:820
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:570
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTAGGTTGCTGGCGAAAG
Suggested primer 2:TCATAATGTGTCGCTGCTCC