| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174210 |
| Chromosome: | chromosome 3 |
| Location: | 540986 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145367 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCCGCGCGCCCCGCCCCGGACCGTATC |
| Internal bar code: | CCGTGAGGGCTGCCGCCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 92.3754 |
| LEAP-Seq n confirming: | 1575 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACCCCATGCACTTACT |
| Suggested primer 2: | ATACCGGTGCTACATCAGCC |