| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174216 |
| Chromosome: | chromosome 9 |
| Location: | 7702540 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415700 | CAH3 | Carbonic anhydrase 3; (1 of 1) PTHR18952//PTHR18952:SF86 - CARBONIC ANHYDRASE // CARBONIC ANHYDRASE-LIKE PROTEIN 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCCAGGTTGGCGGACCCGCAACTTCG |
| Internal bar code: | GATCGGCACAGGGTCCTTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 99.7008 |
| LEAP-Seq n confirming: | 1666 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATACTCCAAGTGCCGGT |
| Suggested primer 2: | CAGTCCCTCTGCCTTTTGAG |