| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174246 |
| Chromosome: | chromosome 3 |
| Location: | 4898619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179550 | TF2H2 | (1 of 1) K03142 - transcription initiation factor TFIIH subunit 2 (TFIIH2, GTF2H2, SSL1); Transcription factor TFIIH, 44 kDa subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGCAGGTGCGGGTTTTGGAGTGTGCAG |
| Internal bar code: | CGGAACAGGTACTGCGAAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.4209 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACACATACACACACAC |
| Suggested primer 2: | AGGTGCGCAGATAAGCAGTT |