Insertion junction: LMJ.RY0402.174259_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.174259
Chromosome:chromosome 1
Location:5653993
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g040300 DNJ9 Catechol O-methyltransferase; (1 of 1) 2.1.1.6 - Catechol O-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATTGGAATAAACTAACACACACACACAC
Internal bar code:ACCGGCCGAATAGATTATGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2
LEAP-Seq percent confirming:35.8586
LEAP-Seq n confirming:213
LEAP-Seq n nonconfirming:381
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAAACGCAACGCTGACTA
Suggested primer 2:GTGGCACTGACTCAGCAGAA