| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174268 |
| Chromosome: | chromosome 9 |
| Location: | 4667572 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396772 | Similar to Gametolysin and Autolysin; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACTGCAAGCTCGCTTGACCGTGACCGC |
| Internal bar code: | CCGACTACTGTGGACCAGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 98.9547 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGGCTGAGATACCGC |
| Suggested primer 2: | CGCATACTTTCAGCGAATGA |