| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174287 |
| Chromosome: | chromosome 16 |
| Location: | 1389875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652100 | MAP2 | (1 of 1) PTHR10804//PTHR10804:SF9 - PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P // METHIONINE AMINOPEPTIDASE 2; Methionine aminopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTCGGACCCTCTCCCAGCGGTTGGCC |
| Internal bar code: | GCAGTTACGTATCTGTTGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.6127 |
| LEAP-Seq n confirming: | 2829 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGATGAGGGATGTCGAGGG |
| Suggested primer 2: | GTGGACCGCAACACCTATCT |