| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174290 |
| Chromosome: | chromosome 1 |
| Location: | 2795189 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016556 | (1 of 1) K03115 - casein kinase II subunit beta (CSNK2B) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGTCCGTGATAGCGTCCTGCTCCTGG |
| Internal bar code: | TACTTAATCCCCGTACGCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.3994 |
| LEAP-Seq n confirming: | 331 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGGGACTTGTGTATGTA |
| Suggested primer 2: | ACAACCCATCCTGCAAAGTC |