| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174290 |
| Chromosome: | chromosome 6 |
| Location: | 2780564 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271050 | URN1 | (1 of 1) 3.2.2.3 - Uridine nucleosidase / Uridine ribohydrolase; Inosine/uridine-preferring nucleoside hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCAGGCCCTGGCCCGTTAGCCAGGCAA |
| Internal bar code: | ACTGGCGCGTAGTTGCGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 99.7422 |
| LEAP-Seq n confirming: | 3869 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTAAGCCAAGAGGACG |
| Suggested primer 2: | TTTACGCGTGTGCAAGCTAC |