Insertion junction: LMJ.RY0402.174298_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.174298
Chromosome:chromosome 10
Location:2134555
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g433500 PWR10 (1 of 1) IPR003590//IPR006502 - Leucine-rich repeat, ribonuclease inhibitor subtype // Protein of unknown function PDDEXK-like; PWR motif protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACGCCACGCAACACCCAACACTCCCCG
Internal bar code:ATCTGGTCCGGTTCCGCAACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:99.5257
LEAP-Seq n confirming:1259
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTTCCAGATGATGGCGTC
Suggested primer 2:TCATCATCCAGTCCTCTCCC