| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174312 |
| Chromosome: | chromosome 3 |
| Location: | 4486025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176150 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCGTATTCGCGCACAGGCGAACTCCG |
| Internal bar code: | GCAGAGTCGTCGAGGATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 93.4569 |
| LEAP-Seq n confirming: | 2471 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATTGCACCAATCACCAC |
| Suggested primer 2: | CCCTGACACACAAATTGCAC |