| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174312 |
| Chromosome: | chromosome 7 |
| Location: | 5310341 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349600 | ISCA2,ISC2 | Putative A-type Fe-S scaffold protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTATAGGCGGTGCTCGTATGCGGTGGA |
| Internal bar code: | AGGTGCGAAGATGCGGTGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 972 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATCCGCCTTTCCTTCT |
| Suggested primer 2: | TTCTGACCTGGCGAACTCTT |