| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174312 |
| Chromosome: | chromosome 7 |
| Location: | 5310342 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349600 | ISCA2,ISC2 | Putative A-type Fe-S scaffold protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGCCGAGACCCTCGCAATGCTCAGCCG |
| Internal bar code: | GTTGAGGTCCACAATTATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.5905 |
| LEAP-Seq n confirming: | 6080 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATCCGCCTTTCCTTCT |
| Suggested primer 2: | TTCTGACCTGGCGAACTCTT |