Insertion junction: LMJ.RY0402.174317_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.174317
Chromosome:chromosome_9
Location:1811380
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g396100 PHC15,EZY16 Cell wall protein pherophorin-C15 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GACTTCTTCGCTGTAAATCTCTTTGATGAA
Internal bar code:CTTTATAGAAAAATTCCCCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:726
LEAP-Seq percent confirming:99.8423
LEAP-Seq n confirming:1266
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCGTCTACAAGGTGTAA
Suggested primer 2:CGAATACCGCAACGGACTAT