Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.174317 |
Chromosome: | chromosome_9 |
Location: | 1811380 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g396100 | PHC15,EZY16 | Cell wall protein pherophorin-C15 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACTTCTTCGCTGTAAATCTCTTTGATGAA |
Internal bar code: | CTTTATAGAAAAATTCCCCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 726 |
LEAP-Seq percent confirming: | 99.8423 |
LEAP-Seq n confirming: | 1266 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCCGTCTACAAGGTGTAA |
Suggested primer 2: | CGAATACCGCAACGGACTAT |