| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174317 |
| Chromosome: | chromosome 9 |
| Location: | 1811380 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396100 | PHC15,EZY16 | Pherophorin-chlamydomonas homolog 15; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTCTTCGCTGTAAATCTCTTTGATGAA |
| Internal bar code: | CTTTATAGAAAAATTCCCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 99.8423 |
| LEAP-Seq n confirming: | 1266 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCGTCTACAAGGTGTAA |
| Suggested primer 2: | CGAATACCGCAACGGACTAT |