| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174317 |
| Chromosome: | chromosome 9 |
| Location: | 1811417 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396100 | PHC15,EZY16 | Pherophorin-chlamydomonas homolog 15; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATGGGTGCTGCGGCCAACATGTTGCAA |
| Internal bar code: | GTTATCCGGTCGCTCGCTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 93.0829 |
| LEAP-Seq n confirming: | 2032 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATACCGCAACGGACTAT |
| Suggested primer 2: | GCCCCGTCTACAAGGTGTAA |