| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174336 |
| Chromosome: | chromosome 9 |
| Location: | 5516078 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400478 | DIV31 | phosphatidylinositol 4-phosphate 5-kinase; (1 of 1) PTHR23086:SF8 - PROTEIN PPK-2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCGGTGCGCCAGCACTGCAAGCCGCA |
| Internal bar code: | ACCGGTTTATTGCAACATATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.6432 |
| LEAP-Seq n confirming: | 4189 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTATGGAAGTGCCTCAC |
| Suggested primer 2: | GGAATTCAATGGCACGTCTT |