| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174370 |
| Chromosome: | chromosome 6 |
| Location: | 5403570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283900 | (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGAACTGGACGAGCGGAAGGAGCACCC |
| Internal bar code: | GTCCGTTCGGCCCAATGCGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 95.9704 |
| LEAP-Seq n confirming: | 207036 |
| LEAP-Seq n nonconfirming: | 8693 |
| LEAP-Seq n unique pos: | 258 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGCGTGTATAACCTGT |
| Suggested primer 2: | GCGCTATCTTTTCCTTGTGC |