| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174378 |
| Chromosome: | chromosome 17 |
| Location: | 6196416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGACTGCTGTACGGCAGCGGCAGCA |
| Internal bar code: | TCGCTGGGCCACCGGTGACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 99.6628 |
| LEAP-Seq n confirming: | 23937 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGCAATCATCCCTTCC |
| Suggested primer 2: | TTGGCGTATCAGATGATGGA |