| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174378 |
| Chromosome: | chromosome 17 |
| Location: | 6196419 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCTCCATCAGCTCCATCACCAGGCACA |
| Internal bar code: | CATCCGTCTCAGACATTGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 98.3873 |
| LEAP-Seq n confirming: | 7992 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCGTGCTCTGACAGTTG |
| Suggested primer 2: | TTGGCGTATCAGATGATGGA |