| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174403 |
| Chromosome: | chromosome 8 |
| Location: | 708215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360350 | ADI1 | Arginine deiminase; (1 of 1) 3.5.3.6 - Arginine deiminase / Arginine dihydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTCCTCTGTACCAATCCTTGCACACAC |
| Internal bar code: | TCATCGTCAGTCACTGTTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGAGAAGGTGGTGAGA |
| Suggested primer 2: | TACGAACTGGCACTAACCCC |