| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174405 |
| Chromosome: | chromosome 2 |
| Location: | 4768412 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102600 | (1 of 1) PTHR23012:SF92 - RING/U-BOX DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGAGCGGCGAGGCCATCTCGCTGCAGT |
| Internal bar code: | TTAAGCCGTAGCAGCCGGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 55.1282 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGTGATGTACCCAGGAC |
| Suggested primer 2: | CACACACACCAGTCTACCCG |