Insertion junction: LMJ.RY0402.174411_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.174411
Chromosome:chromosome 11
Location:1868154
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g468050 VIPP2 (1 of 2) K03969 - phage shock protein A (pspA); Vesicle inducing protein in plastids 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACAACGGTGCAAGTTGGACCAGCAGCA
Internal bar code:TTCGGTGAGCTGCCCTGAACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:99.6774
LEAP-Seq n confirming:309
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCCTCGTTCTCACCTGC
Suggested primer 2:CTTCTTCAACGGCCTAGTCG