| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174414 |
| Chromosome: | chromosome_2 |
| Location: | 3239692 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g095076 | MFT10 | Major facilitator superfamily transporter, involved in circadian | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTATTTTTATTTGATAGCAACGCACGGTGC |
| Internal bar code: | GCGCATCGCAGTCGCAGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 79.7896 |
| LEAP-Seq n confirming: | 1062 |
| LEAP-Seq n nonconfirming: | 269 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGCGTTTCGTTCTTAAC |
| Suggested primer 2: | ATGGGCACTCCTTCGTACAC |