| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174432 |
| Chromosome: | chromosome 17 |
| Location: | 4254312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTCTTGTGCCGGCTTATCGTTGCGCGT |
| Internal bar code: | CGGTGTTCCGCTGAAGTATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 96.8433 |
| LEAP-Seq n confirming: | 859 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCTGCCTTATTAGCGG |
| Suggested primer 2: | CTGAACATGAACGGGACCTT |