| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174447 |
| Chromosome: | chromosome 3 |
| Location: | 688437 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145967 | (1 of 2) K15437 - aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1, ARC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGGATGTGTGCTGGGGAGCGAAAGTC |
| Internal bar code: | CATCCGGATTTCAGACCTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 94.7917 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGACGGGTTTCCATTGTC |
| Suggested primer 2: | CCTGTTCCCCGATATGAAGA |