| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174447 |
| Chromosome: | chromosome 8 |
| Location: | 622634 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359850 | FBB11 | Flagellar and Basal Body Protein 11; (1 of 2) PF13864 - Calmodulin-binding (Enkurin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTGGTGATGGGGGAAGACGGGGCACA |
| Internal bar code: | TCCCGGCGGGAGGCCCTCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 81.3441 |
| LEAP-Seq n confirming: | 1513 |
| LEAP-Seq n nonconfirming: | 347 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACGGAGAAGTCGTGCC |
| Suggested primer 2: | ACTGAGTGGACAACCCTTGG |