Insertion junction: LMJ.RY0402.174450_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.174450
Chromosome:chromosome 3
Location:8038901
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGGCAACGCGGTACTGCCCACGGGGCC
Internal bar code:TAGTGTCTTTGAATTGCAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:847
LEAP-Seq percent confirming:94.382
LEAP-Seq n confirming:168
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCTCCACCTCTGTCAAG
Suggested primer 2:ACTCGTCACATCTCAGGGCT