| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174454 |
| Chromosome: | chromosome 8 |
| Location: | 3925510 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378400 | 3'UTR | ||
| Cre08.g378417 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCCATCCATCACTGCGGATGTGTGGCT |
| Internal bar code: | TTTTGGGCTTTTATCGCGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 99.3817 |
| LEAP-Seq n confirming: | 1768 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTTAGGCAGAGGAGGA |
| Suggested primer 2: | GGGTCCGGACTCTCATTGTA |