| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174460 |
| Chromosome: | chromosome 17 |
| Location: | 7069011 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746997 | ADHE,ADHE1,ADH1 | Dual function alcohol/acetaldehyde dehydrogenase; (1 of 2) 1.2.1.10 - Acetaldehyde dehydrogenase (acetylating) / Aldehyde dehydrogenase (acylating) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCGACGTGTGCCTGTAAGTACCTGA |
| Internal bar code: | GTGCACATGTACAAAGTGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 87.2126 |
| LEAP-Seq n confirming: | 2162 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGAGGCGCGGTACTTAG |
| Suggested primer 2: | ACCCGCGCTGATGTATAAAG |