Insertion junction: LMJ.RY0402.174487_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.174487
Chromosome:chromosome 9
Location:1108200
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g400312 (1 of 5) IPR001680//IPR011047//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATCCTAACGAACCTCCTGCCCGAGTTT
Internal bar code:CTGCGGGTTAGGATCCGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:310
LEAP-Seq percent confirming:98.0545
LEAP-Seq n confirming:252
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGTGTGAAGCATACCTTT
Suggested primer 2:GTAAGTCCAGCCTTTGCTGC