| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174490 |
| Chromosome: | chromosome 16 |
| Location: | 4410476 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671200 | BLZ20 | bZIP transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGCCGAGCCGCGCCGCTTGTATGCAGG |
| Internal bar code: | GAATGGGCGGAAGGCCGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 90.6266 |
| LEAP-Seq n confirming: | 1750 |
| LEAP-Seq n nonconfirming: | 181 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCCTGGTGACCTGTT |
| Suggested primer 2: | GGTGCCGTACGAATGTCTTT |