| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174528 |
| Chromosome: | chromosome 9 |
| Location: | 4486685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395769 | FAP177 | Flagellar Associated Protein 177 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCCAAGTTGCAGCGGGCGGGATGGCG |
| Internal bar code: | TCGGCAAGCAGTTAATGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 91.7955 |
| LEAP-Seq n confirming: | 772 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGACGGAAGTGGTGTCC |
| Suggested primer 2: | AGGCTGACCAGAGCAGTGAT |