| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174534 |
| Chromosome: | chromosome 6 |
| Location: | 7246090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298225 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTACAGCCACCGCCGAGTGCTGGAGCA |
| Internal bar code: | AGTACGTCCGGAGATCCACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 94.6012 |
| LEAP-Seq n confirming: | 771 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGTTTTAGCAATGTGCC |
| Suggested primer 2: | ACACTCACCCCAAAGTCCTG |