| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174536 |
| Chromosome: | chromosome 12 |
| Location: | 9337245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540650 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCAGTTCGACGCGCACAACCCCCAGG |
| Internal bar code: | ATCAGGCCTGGGGGATGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.0644 |
| LEAP-Seq n confirming: | 953 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCAGCAACCACACTCA |
| Suggested primer 2: | GGATGTTTGGAAAAGCGGTA |