| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174567 |
| Chromosome: | chromosome_6 |
| Location: | 7761839 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g302200 | EFH3 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCGGGCGCCAGGGCTCGGCGTGGAGCAG |
| Internal bar code: | TAAATCTGCATCCATGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.8219 |
| LEAP-Seq n confirming: | 3363 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAAACAGTATGAACGCC |
| Suggested primer 2: | ACTATGGCCCAGATGACGAC |