| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174568 |
| Chromosome: | chromosome 5 |
| Location: | 1744905 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242650 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCAACTCTGGGGCTCGTCAACCCCACAC |
| Internal bar code: | ACGCAAATGAAAGCCGTGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 99.5556 |
| LEAP-Seq n confirming: | 2912 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGTTGCCAAACCAGTC |
| Suggested primer 2: | GCACTGAACACACATCCCAC |