| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174590 |
| Chromosome: | chromosome 10 |
| Location: | 954252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424700 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGAATACATGAGGAGCGTCCTATGGGA |
| Internal bar code: | TCGTGAAGTACAGTGTTCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 98.9513 |
| LEAP-Seq n confirming: | 6039 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGCTGTCAAAATGGTG |
| Suggested primer 2: | GAGCCGTGTAGCACCTTCTC |