| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174598 |
| Chromosome: | chromosome 4 |
| Location: | 3664003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228850 | (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGGAAGCTGGATGACTACGACATCC |
| Internal bar code: | ATGGAAGGACGGAAGGAGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 94.8412 |
| LEAP-Seq n confirming: | 12336 |
| LEAP-Seq n nonconfirming: | 671 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGGAAGAGAGGGAG |
| Suggested primer 2: | TAGCGACTGGCAAGAATGTG |