| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174610 |
| Chromosome: | chromosome 14 |
| Location: | 3759773 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632350 | FAP138 | Coiled-Coil Flagellar Associated Protein 138 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCTTCGCACACATAGCAGTATCCCGA |
| Internal bar code: | CTAATCTCGAAGTCCCCCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 93.0876 |
| LEAP-Seq n confirming: | 202 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGATATTGGTGCAGCCC |
| Suggested primer 2: | CAGACGACATGCACATAGGG |