| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174617 |
| Chromosome: | chromosome 4 |
| Location: | 2823310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224150 | (1 of 1) 2.7.1.30 - Glycerol kinase / Glycerokinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGAATACAGCACCATCAAGGACGGCAC |
| Internal bar code: | CTGCCCTGTGAAGATGGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.7818 |
| LEAP-Seq n confirming: | 4116 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTTTCACATTGGCTG |
| Suggested primer 2: | CTGGGCCAGTTCACCTGTAT |