| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174633 |
| Chromosome: | chromosome 7 |
| Location: | 144400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313164 | (1 of 2) K16296 - serine carboxypeptidase-like clade I [EC:3.4.16.-] (SCPL-I) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGACCTGACACCCAGCCCAAGCTCCCTG |
| Internal bar code: | AGGTTTTCGACATTGATCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.5875 |
| LEAP-Seq n confirming: | 4346 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCTTGCTTCAAGCTGTC |
| Suggested primer 2: | CATTGTGTTCTCAACCACCG |