| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174645 |
| Chromosome: | chromosome 10 |
| Location: | 1239861 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426650 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTTGTGCTTCCTCCGGGAGCCCGATGG |
| Internal bar code: | AGTTGTACGTCCTCGGTGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 78.6609 |
| LEAP-Seq n confirming: | 2890 |
| LEAP-Seq n nonconfirming: | 784 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGTACACGTCCTCAAAA |
| Suggested primer 2: | TGGTGAGCAGTGGTCTGAAG |