Insertion junction: LMJ.RY0402.174651_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.174651
Chromosome:chromosome 6
Location:1751687
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGGTGTAGCGGCAGCGCAGCACAAGCT
Internal bar code:AGTACTCAGTCGACACTTGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:674
LEAP-Seq percent confirming:99.1465
LEAP-Seq n confirming:2091
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCAGGTAGGACTTCACC
Suggested primer 2:GATGGAAAAGGTGCTTTGGA