| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174657 |
| Chromosome: | chromosome 10 |
| Location: | 1767227 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430750 | (1 of 1) PTHR24078//PTHR24078:SF261 - DNAJ HOMOLOG SUBFAMILY C MEMBER // DNAJ (HSP40) HOMOLOG, SUBFAMILY A, MEMBER 3A | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGACGGCGAGCAAAAGCGGAGCTGC |
| Internal bar code: | TCTCTACGCCTCGGTTATTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1122 |
| LEAP-Seq percent confirming: | 99.6562 |
| LEAP-Seq n confirming: | 11884 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCCTAGAATGGAAGTGA |
| Suggested primer 2: | ACGCTTACCGAGTCCTCTGA |