| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174662 |
| Chromosome: | chromosome 2 |
| Location: | 18337 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073150 | (1 of 1) IPR001841//IPR029071 - Zinc finger, RING-type // Ubiquitin-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCTATCAAAGATTTGAGGGATTGTTTT |
| Internal bar code: | TTTCCATTTCAGCTTCTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.4966 |
| LEAP-Seq n confirming: | 593 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATCTGCAGAGCCAGGAA |
| Suggested primer 2: | TCAACGGTAAGCAGTGCAAG |