| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174664 |
| Chromosome: | chromosome 1 |
| Location: | 6823788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049200 | (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAAGCGCGTGGAGATGACGGCCGTTGG |
| Internal bar code: | TTACGACAGGGTACGGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 98.531 |
| LEAP-Seq n confirming: | 1811 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCCGTTTGTCCACTGTA |
| Suggested primer 2: | GGCTCAAAGTCAACGAGGAG |