| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174678 |
| Chromosome: | chromosome 7 |
| Location: | 1942681 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325748 | (1 of 1) 3.5.2.9 - 5-oxoprolinase (ATP-hydrolyzing) / Pyroglutamase (ATP-hydrolyzing) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACATATTGCAAACGTGATTCACAACATG |
| Internal bar code: | GGGTATGAATGTTGGGGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACCTGTGACCTCCTCC |
| Suggested primer 2: | CTAAACGCTCCACCCAACAT |