| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174686 |
| Chromosome: | chromosome 12 |
| Location: | 1480547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487600 | CPS2 | Putative cleavage and polyadenylation specificity factor 100 kDa subunit; (1 of 1) K14402 - cleavage and polyadenylation specificity factor subunit 2 (CPSF2, CFT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCATCGCAGCCCGACCCGCCAACCTC |
| Internal bar code: | GTTCTCCGAAACGGTTCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 97.6149 |
| LEAP-Seq n confirming: | 3356 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGCTGCCTATTCCTGAG |
| Suggested primer 2: | ATGGCTACTTGCAAGGGATG |